Abstract：【Objective】To investigate the mechanism of Tanshinone ⅡA inhibition of MPP+ induced SH-SY5Y cell oxidative stress. 【Methods】Oxidative stress of SH-SY5Y cells was induced by MPP+ treatment. Tanshinone ⅡA intervention and Znpp inhibition of HO-1 expression were measured. MTT assay was used to detect the cells viability. Oxidative stress indices including superoxide dismutase (SOD) and malondialdehyde (MDA) in cells were applied via test kit. HO-1 mRNA and protein expressions were detected by real-time PCR and Western Blot. The experimental designs were: the control group (routine culture with vehicle), MPP+ group (1 mM MPP+), MPP+ + Tanshinone IIA group (1 mM MPP+ + 5 ug/mL Tanshinone IIA), and Znpp + MPP + Tanshinone IIA group (5 μM Znpp pretreatment +1 mM MPP+ + 5 ug/mL Tanshinone IIA).【Results】Compared to the control group, the cells viability and SOD level of MPP+ group was lower (P<0.05), and the MDA level was higher (P<0.05). Compared with the MPP+ group, the cells viability of MPP+ + Tanshinone ⅡA group was higher, the level of SOD increased while the MDA level decreased (P<0.05). The expressions of HO-1 mRNA and protein in the MPP+ group were higher than those in the control group (P<0.05),while the expression of HO-1 was further increased after tanshinone ⅡA treatment. The protective effects of tanshinone Ⅱ A on cell viability and oxidative stress were eliminated by HO-1 inhibitor Znpp. 【Conclusion】Tanshinone ⅡA can attenuate oxidative stress damage induced by MPP+ in SH-SY5Y cells, which may be related to the induction of HO-1 expression.
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2018, Vol. 35 
