Abstract：【Objective】To evaluate the predictive value of urine α-1 microglobulin (α1-MG) level for preeclampsia (PE) during gestational rats.【Methods】A total of 26 female SD rats under SPF condition were confirmed to be pregnant. These SD female rats were randomly divided into the PE model group and the normal pregnancy group, with 13 in each group. From the 11th day of pregnancy, rats in PE model group received Angiotensin Ⅱ-type 1 receptor antagonist (AT1-AA) while mice in normal pregnancy group received equal volume of saline by tail vein injection for 7 days. The tail artery systolic pressure was measured by animal's noninvasive blood pressure tester from the 10th day of pregnancy. The urine of pregnant rats of the two groups was collected on the 8th, 12th, 16th, and 20th day of pregnancy. The concentration of urine α1-MG in the two groups was detected by rate immunoassay. Caesarean section was performed before pregnancy end. The average number of newborn rats, the rate of deformity and stillbirth rate were compared between the two groups. The weight of newborn rats and wet weight of placenta were measured.【Results】At 12 to 21 days of gestation, the systolic blood pressure of the tail artery in the PE model group was significantly higher than that of the normal pregnancy group (P<0.05). The systolic pressure of the tail artery in the PE model group increased significantly from the 12th to the 18th day of gestation, and there was a statistically significant difference between the two adjacent days (P<0.05). The level of α1-MG in urine of d12, d16and d20during the gestational period was significantly higher in the PE model group than in the normal pregnancy group (P<0.05). The difference between the urinary α1-MG levels in the PE model group was statistically significant (P<0.05). The average number and weight of newborn rats and wet weight of placenta in PE model group were significantly lower than those in the normal pregnancy group (P<0.05). The deformity rate and stillbirth rate in the PE model group were significantly higher than those in the normal pregnancy group (P<0.05).【Conclusion】Pregnancy hypertension, higher levels of urinary α1-MG, and poor quality of newborn rats are observed in the AT1-AA induced PE rat model. So, it is speculated that the detection of urine α1-MG level has important predictive value for preeclampsia in rats.
刘瑀晗,包南丁,张越,张艳宁. 尿α1微球蛋白对妊娠期大鼠子痫前期的预测价值[J]. 医学临床研究, 2018, 35(3): 441-444.
LIU Yu-han, BAO Nan-ding, ZHANG Yue, et al. Urine α-1 Microglobulin Level in Prediction of Preeclampsia in Pregnant Rats. JOURNAL OF CLINICAL RESEARCH, 2018, 35(3): 441-444.
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2018, Vol. 35 
