Abstract：【Objective】To investigate the effect of dexmedetomidine (Dex) on hepatic ischemia reperfusion injury (IRI) in cholestatic rats.【Method】A total of 24 cholestatic rats modeled by bile duct ligation were randomly divided into 3 groups (n=8): sham operated group (A group), hepatic IRI group (B group) and Dex group (C group). The hepatic portal triad were clamped by Pringle maneuver and maintained for 30 minutes. After 4 hours of reperfusion, blood samples were obtained for testing levels of total bilirubin (TBIL), direct billirubin (DBIL), alanine transaminase (ALT), aspartate transaminase (AST), and tumor necrosis factor-α (TNF-α). Liver tissues homogenates were obtained to test values of superoxide dismutase (SOD) and malondialdehyde (MDA), and were observed after hematoxylin-eosin (HE) staining for histopathological assessment.【Results】After 4 hours of reperfusion, TBIL and DBIL levels in three groups were not significantly different (P>0.05). Serum ALT, AST and TNF-α levels, tissue MDA contents in the B group were significantly higher than those in the A group (P<0.05). Tissue SOD activities in the B group were significantly lower than the A group (P<0.05). Serum AST and TNF-α levels, tissue MDA contents in the C group were significantly lower than the B group (P<0.05). Histopathological changes (liver tissue destruction, hepatic cord disorders and inflammatory cell infiltration) in the B group were more obvious than in the A and C groups.【Conclusion】Dexmedetomidine may attenuate hepatic IRI injury in cholestatic rats, possibly by alleviating systemic inflammation and oxidative stress.
龙迎曦,邹毅,陈文雁,黄红珏,谌思,孔高茵. 右美托咪定对胆汁淤积大鼠肝脏缺血再灌注损伤的影响[J]. 医学临床研究, 2018, 35(3): 425-427,431.
LONG Ying-xi, ZOU Yi, CHEN Wen-yan, et al. Effect of Dexmedetomidine on Hepatic Ischemia Reperfusion Injury in Cholestatic Rats. JOURNAL OF CLINICAL RESEARCH, 2018, 35(3): 425-427,431.
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2018, Vol. 35 
