Abstract：【Objectives】To explore the expression of COX-2 and Ki-67 in patients of hepatobiliary calculus with Cholangiocarcinoma (HCWC) and their clinical significance. Relationship between the expression of COX-2、Ki-67 and the onset and progress of HCWC was investigated to lay an experimental base for the prevention and treatment of HCWC.【Methods】Eighteen cases (n=18) of normal bile duct tissue from operations of hemangiomas of liver or liver trauma were used as the control (group A);fifty-five (n=55) cases of the bile duct tissue surrounding calculus of intra-hepatic were selected as group B, and forty-four (n=44) cases of tumor tissue of HCWC (group C) were randomly sampled and collected between January 2000 to December 2016 in the Department of Hepatobiliary Surgery of Hunan Provincial People's Hospital. Immunohistochemistry was used to detect the expression of COX-2 and Ki-67 as well as their expression in cholangiocarcinoma in correlation with clinicopathological features. The results were statistically analyzed.【Results】In group A,B,C, the positive rates of the expression of COX-2 and Ki-67 were 5.56%,32.72%, 70.45%, and 0, 27.27%,79.55%, respectively. The positive rate of the expression of COX-2 and Ki-67 in the carcinoma tissue of HCWC was significantly higher compared with the control group (P＜0.05); the positive rate of COX-2 and Ki-67 expression in poorly differentiated HCWC cases was higher compared with highly differentiated cases (P＜0.05); the positive rate of COX-2 and Ki-67 in extra-hepatic or lymphatic metastasis HCWC cases was higher compared with cases without metastasis (P＜0.05). There was no significant difference in the expression rate of COX-2 and Ki-67 between the different age groups, sex, duration, tumor size and pathological type of group C (P＞0.05). The positive expression rate of Ki-67 in COX-2 positive cases of group C was 90.32% (28/31). Spearman-related analysis showed a strong positive correlation between the expression of COX-2 and Ki-67 in HCWC(r=0.413,P=0.005).【Conclusions】 There is a significantly positive correlation between the expression of COX-2 and Ki-67 in HCWC. The high expression of COX-2 and Ki-67 play a certain role in the occurrence and progress of HCWC.
陈晨,杨熙雯,罗良,蒋波,李浩,汪新天,王群伟. COX-2、Ki-67在肝胆管结石并胆管癌组织中的表达及其与临床病理特征的相关性[J]. 医学临床研究, 2017, 34(9): 1729-1732.
CHEN Chen, YANG Xi-wen, LUO Liang, JIANG Bo,et al. Study of COX-2 and Ki-67 Expression in Hepatobiliary Calculus with Cholangiocarcinoma. JOURNAL OF CLINICAL RESEARCH, 2017, 34(9): 1729-1732.
[1]王云,陈敏,陈骏,李运红,诸葛宇征.Claudin-4在胆管癌中的表达及其临床意义[J].胃肠病学,2016,21(8):461-464. [2]许良中,杨文涛.免疫组织化学反应结果的判断标准[J].中国癌症杂志,1996,6(4):229-232. [3]吴金术,朱朝庚,易为民,等.肝内胆管结石并胆管癌的诊治体会(附18例报告) [J].肝胆外科杂志,2001,9(6):427-429. [4]郜朝霞,孙权.环氧合酶-2在胆管癌中的研究进展[J].国外医学.外科学分册,2005,13(3):187-190. [5]蒋安科,李强,李博,等.CA19-9和CEA在肝胆管结石合并胆管癌诊断与预防评估中的价值[J].肝脏,2015,20(11):862-864. [6]黎军,卓士超.COX-2和Ki-67在人脑胶质瘤中的表达和临床意义[J].齐齐哈尔医学院学报,2009,30(8).911-912. [7]Derya G,Seyda E,Fazilet K,et al.Expression of COX-2,PCNA,Ki-67 and p53 in gastrointestinalstromal tumors and its relationship with histopathologicalparameters[J].World J Gastroenterol,2007,13(3):426-431. [8]JeonHG,JeongIG,BaeJ,etal.Expression of Ki-67 and COX-2 in patients with upper urinary tract urothelial carcinoma[J].Urology,2010,76(2):7-11
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