Abstract：【Objective】To investigate the relationship between KCNJ11 promoter methylation and insulin levels in patients with type 2 diabetes mellitus (T2DM).【Methods】From April 2014 to April 2015, 15 patients with T2DM initially treated in our hospital were selected. Those HbA1c＞9% were enrolled in the blood-sugar severely elevated group (group A, n=5),and those HbA1c ≤9% were enrolled in the blood-sugar mildly elevated group (group B,n=10); 15 cases of normal physical examination at the same period were enrolled in the control group (group C); The DNA methylation level of 46 CpG sites in the 5 'end 300 bp of the KCNJ11 gene promoter was determined by the sulfite method.【Results】The BMI in the group A was higher than that in the group B and the group C, and the difference was significant (P＜0.05); There was a significant difference in fasting blood glucose between the three groups (P＜0.05);HbA1c was significantly different between the three groups in the one-one contrast (P＜0.05); FIns comparison between the three groups had obvious difference (P＜0.05); CpG+62, A, CpG+66, CpG+351, CpG+62 + CpG+66 methylation rate of Group A was lower than those of Group B and Group C, and Group B was lower than Group C, there were statistical differences (P＜0.05); The methylation rates of 3 methylation sites in Group A and Group B were negatively correlated with FINS.【Conclusion】The methylation of KCNJ11 promoter region was negatively correlated with insulin levels in type 2 diabetes mellitus patients.
李雯翀,朱咏瑶,刘抐岢,陈毅光. 新发2型糖尿病患者KCNJ11启动子区甲基化与胰岛素相关性分析[J]. 医学临床研究, 2017, 34(8): 1457-1460.
LI Wen-chong, ZHU Yong-yao, LIU Rui-ke,et al. Association of KCNJ11 Promoter Methylation with Insulin in Patients with Newly Diagnosed Type 2 Diabetes Mellitus. JOURNAL OF CLINICAL RESEARCH, 2017, 34(8): 1457-1460.
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