Abstract：【Objective】 To investigate the expression of IL-13 (interleukin-13) and its effect on inflammatory reaction of acute lung injury (ALI) induced by LPS in mice.【Methods】LPS was intratracheally injected into mice to induce the ALI model. The real-time PCR and ELISA were used to detect IL-13 mRNA and protein expression in lung tissue, bronchoalveolar lavage fluid (BALF) and serum. The effects of IL-13 on ALI was investigated using IL-13 neutralizing antibody. The ELISA method was used to detect the content of TNF-α and IL-1β in BALF and serum. HE staining was used to observe pathological damage of lung tissue changes in mice.【Results】Compared with the control group, IL-13 mRNA expression in lung tissue and IL-13 protein expression in BALF and serum were increased markedly in ALI mice induced by LPS (P<0.05). Pretreatment of IL-13 neutralizing antibody increased the expression of TNF-α and IL-1β, and aggravated the changes in pathological damage of ALI mice (P<0.05).【Conclusion】 IL-13 expression increases in ALI induced by LPS. While inhibition of IL-13 aggravates the damage in mouse with ALI induced by LPS, which indicates that IL-13 plays a protective role in ALI endogenously.
曹健斌. IL-13在急性肺损伤小鼠肺内的表达及其意义研究[J]. 医学临床研究, 2017, 34(6): 1087-1089.
CAO Jian-bin. Expression and Role of IL-13 in Mice with Acute Lung Injury. JOURNAL OF CLINICAL RESEARCH, 2017, 34(6): 1087-1089.
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2017, Vol. 34 
