Abstract：【Objective】To explore the ability of ectopic bone formation of osteoblastic-induced hUCMSCs composite with nHA/PA66 material in nude mouse back and provide theories foundation for further large animal experiments of bone tissue engineering.【Methods】Human umbilical cord mesenchymal stem cells were seeded into nHA/PA66 scaffolds and cultured in vitro; HUCMSCs suspension, nHA/PA66 scaffold material, osteogenic-induced hUCMSCs and nHA/PA66 scaffold composite were implanted into the dorsal muscle pouch of nude mice; the ectopic bone formation was observed. The expression of HE staining and osteocalcin immunohistochemical staining were observed after operation, and the osteogenic ability was observed by scanning electron microscope.【Results】Neither osteoblastic-induced hUCMSCs nor nHA/PA66 material were sclerotized in the muscular pouch of nude mouse back. The osteoblastic-induced hUCMSCs + nHA/PA66 were maturely sclerotized in large amount,the material partly degradated.【Conclusion】 The biological properties of the composites prepared by the combination of hUCMSCs and nHA/PA66 scaffolds in the nude mice have ectopic osteogenesis.
陈刚;廖前德;胡优威;钟达;. 成骨诱导人脐带间充质干细胞复合nHA/PA66支架异位成骨作用的实验研究[J]. 医学临床研究, 2017, 34(4): 666-670,672.
CHEN Gang, LIAO Qian-de, HU You-wei,et al. Study on Ectopic Bone Formation of Osteoblastic-Induced hUCMSCs Composite with nHA/PA66 Material. JOURNAL OF CLINICAL RESEARCH, 2017, 34(4): 666-670,672.
[1] 陈刚,廖前德,钟达,等.成骨诱导人脐带间充质干细胞与nHA/PA66支架材料生物相容性[J].中国组织工程研究, 2012,16 (16):2931-2934. [2] 高健伟.人脐带间充质干细胞治疗脊髓损伤的研究进展[J].中国矫形外科杂志,2013,21(6):582-586. [3] Roberta T.Development of sarcomas in mice implanted with mesenchymal stem cells seeded onto bioscaffolds [J].Carcinogenesis Oxford,2009,30(1):150-157. [4] Sharma M,Afrin F,Satija N,et al.Stromal-derived factor-1/CXCR4 signaling: indispensable role in homing and engraftment of hematopoietic stem cells in bone marrow[J].Stem Cells Dev,2011,20(6):933-946. [5] Dong J,Uemura T,Shirasaki Y,et al.Promotion of bone formation using highly pure porous β-TCP combined with bone marrow-derived osteoprogenitor cells[J].Biomaterials,2002,23(23):4493-4502. [6] Gronthos S,Zannettino AC,Hey SJ,et al.Molecular and cellular characterization of highly purified stromal stem cells derived from human bone marrow[J].J Cell Sci,2003,116(Pt9):1827-1835. [7] 李锦华,王伟.复合BMSCs的异种骨移植材料异位成骨研究[J].武汉大学学报(医学版),2012,33(3):355-358. [8] Liu YS.The effect of simvastatin on chemotactic capability of SDF-1 alpha and the promotion of bone regeneration[J].Biomaterials,2014,35(15): 489-498. [9] 朱彦丞,郑欣.异位成骨动物模型的研究进展[J].中国矫形外科杂志,2015,23(6):524-526. [10] Scott MA,Levi B,Askarinam A,et al.Brief review of models of ectopic bone formation[J].Stem Cells Dev,2012,21:655-667. [11] Metzler P,von Wilmowsky C,Zimmermann R,et al.The effect of current used bone substitution materials and platelet-rich plasma on periosteal cells by ectopic site implantation:an in-vivo pilot study[J].J Craninomaxillofac Surg,2012,40:409-415. [12] 魏冀荣,章莹.骨移植支架材料的理论研究与临床应用[J].中国组织工程,2012,16(47):8880-8884. [13] 施咏毅,王根林,杨惠林,等.多孔型丝素蛋白/羟基磷灰石复合脂肪间充质干细胞修复兔股骨缺损[J].中国组织工程研究与临床康复,2010,14 (8):1341-1344. [14] Wang H,Zhi W,Lu X,et al.Comparative studies on ectopic bone formation in porous hydroxyapatite scaffolds with complementary pore structures[J].Acta Biomater,2013,9:8413-8421. [15] Kumar JP,Lakshmi L,Jyothsna V,et al.Synthesis and characterization of diopside particles and their suitability along with chitosan matrix for bone tissue engineering in vitro and in vivo[J].J Biomed Nanotechnol,2014,10(6):970-981. [16] 林辉,王修超.大鼠骨髓间充质干细胞复合n-HA/PLA支架异位成骨的实验研究[J].中国矫形外科杂志,2012,20:1510-1512. [17] Wang GX,Hu L,Hu HX,et al.In vivo osteogenic activity of bone marrow stromal stem cells transfected with Ad-GFP-Hbmp-2[J].Genet Mol Res,2014,13:4456-4465. [18] 尹科,廖前德,钟达,等.富血小板血浆诱导犬骨髓间充质干细胞的体外成骨 [J] .中国组织工程研究与临床康复,2009,13(49):9697-9700. [19] Park JC,Lee JB,Daculsi G,et al.Novel analysis model for implant osseointergration using ectopic bone formation via the recombinant human bone morphogenetic protein-2/macroporous biphasic calcium phosphate block system in rats:a proof-of-concept study[J].J Periodontal Implant Sci,2012,42:136-143. [20] Oprenyeszk F,Sanchez C,Dubuc JE,et al.Chitosan enriched three-dimensional matrix reduces inflammatory and catabolic mediators production by human chondrocytes[J].PloS One,2015,10(5):e0128362. (本文编辑:詹道友) [收稿日期] 2016-05-03
2017, Vol. 34 
