Abstract：【Objective】To study the effect of sivelestat on the expression of inflammatory factors, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in rats with severe acute pancreatitis. 【Methods】A total of 30 SD rats were divided into control group, model group and intervention group. Rats in the control group underwent a ventral midline incision to exposure pancreas and duodenum without clamping. Severe acute pancreatitis (SAP) was established in the model group and the intervention group by retrograde injection of 5% sodium taurocholate from the duodenal papilla into the biliopancreatic duct. The control group and model group were injected with saline solution by jugular vein catheter, and the intervention group received the sivelestat treatment. The levels of serum inflammatory factors (IL-2, IL-6 and IL-8), ALT and AST were measured at 6h and 12h after SAP initiation. At the same time points, the pathological score of pancreatic tissue was evaluated. 【Results】At 6h and 12h after SAP initiating, the pathological score of pancreatic tissue and serum levels of amylase (AMY), ALT, AST, IL-2, IL-6 and IL-8 in the model group rats were significantly higher than those in the control group(t=10.129~21.664, P<0.01). However, the pathological score of pancreatic tissue and serum levels of AMY, ALT, AST, IL-2, IL-6 and IL-8 in the intervention group were significantly lower than those in the model group (t=6.055~12.551,P<0.01). 【Conclusion】Sivelestat can alleviate pancreatic damage of severe acute pancreatitis in rat, inhibit inflammation, and protect liver function.
陶佳丽;罗曼. 西维来司钠对重症急性胰腺炎大鼠炎性因子及ALT、AST表达的影响[J]. 医学临床研究, 2017, 34(3): 490-493,495.
TAO Jia-li, LUO Man. Effect of Sivelestat on the Expression of Inflammatory Factors, ALT and AST in Rats with Severe Acute Pancreatitis. JOURNAL OF CLINICAL RESEARCH, 2017, 34(3): 490-493,495.
[1] 郝一鸣,王士琪,冯全新,等. 脂肪肝诊断联合APACHE-Ⅱ评分提高预测重症急性胰腺炎的能力[J].中华普通外科杂志,2016,31(1):23-26. [2] 李春燕,廖东平,李仕强,等.丙氨酰－谷氨酰胺对重症急性炎患者IL-6、TNF-α、D-二聚体的影响及临床意义[J].胃肠病学和肝病学杂志,2015,24(3):351-352. [3] Raraty MG, Connor S, Criddie DN, et al. Acute pancreatitis and organ failure pathophysiology, natural history,and management strategies[J].Curr Gas Rep,2012, 6(2): 99-103. [4] 脱红芳,王京斌,郭怀斌,等. 西维来司钠对大鼠重症急性胰腺炎炎症反应的治疗作用[J].世界华人消化杂志,2011,19(35):3579-3584. [5] Wang H, Wang S, Tang A, et al. Combined effects of sivelestat and resveratrol on severe acute pancreatitis-associated lung injury in rats[J].Exp Lung Res,2014, 40(6): 288-297. [6] Barbeiro DF, Koike MK, Coelho AM, et al. Intestinal barrier dysfunction and increased COX-2 gene expression in the gut of elderly rats with acute pancreatitis [J].Pancreatology,2016,16(1):52-56. [7] 汤小龙,向正国,李科军,等.乌司他丁联合奥曲肽治疗急性胰腺炎的疗效及对因子和淀粉酶的影响[J].海南医学院学报,2014,20(12):1639-1641. [8] 王琦,沈世强,林福生,等.西维他司钠对重症急性胰腺炎大鼠肝细胞凋亡及B－淋巴细胞/白血病-2/bax表达的影响[J].中华实验外科杂志,2012,29(6):1127-1130. [9] 王一贺,杨红明,胡泉,等.西维来司钠对重度烧冲复合伤犬急性肺损伤的影响[J].中华烧伤杂志,2014,30(2):158-165. [10] 李科军,向正国,陈旭峰,等.早期大剂量乌司他丁联合奥曲肽治疗对重症急性胰腺炎患者炎症因子和T淋巴细胞亚群的影响[J].海南医学院学报,2015,21(7):929-932. [11] Pooran N, Indaram A, Singh P, et al. Cytokines: Early and reliable predictors of severe acute pancreatitis[J].J Clin Gastroenterol,2011, 37(3) : 263-266. [12] 郭浚,胡刚,曹梦娟.PCT、hs-CRP及IL-6的水平变化对急性胰腺炎的临床意义[J].胃肠病学和肝病学杂志,2014,23(12):1471-1474. [13] 王后红,李国刚,李超,等.西维来司钠对急性坏死性胰腺炎肺损伤保护作用及其机制研究[J].中华急诊医学杂志,2016,25(1):63-66. [14] Wang HH, Tang AM, Chen L, et al. Potential of sivelestat in protection against severe acute pancreatitis-associated lung injury in rats [J].Exp Lung Res,2012, 38(9-10):445-452. [15] 赵宝川,脱红芳,彭彦辉,等. 西维来司钠对大鼠重症急性胰腺炎后急性肝损伤的保护作用[J].天津医药,2011,39(5):458-460. [16] 王琦,沈世强,林福生,等.西维来司钠对重症急性胰腺炎大鼠肝损害的保护作用及其机制[J].中华实验外科杂志,2012,29(5):900-902.
2017, Vol. 34 
