Abstract：【Objective】To explore the effects of growth hormone(GH)on follicular development in patients with decreased ovarian reserve(DOR)under in-vitro fertilization-embryo transfer (IVF-ET).【Methods】A total of 20 DOR patients received IVF-ET therapy were divided into observation group (11 cases, GH) and control group (9 cases, without GH) according to the wishes of patients. On the day of oocyte retrieval, follicular fluid of single dominant follicle (diameter ≥16 mm) of the two groups was collected , and by Western Blot method, semi quantitative detection of each follicle insulin-like growth factor -1 (IGF-1), IGF-1 receptor (IGF-1R) and GH were conducted; and their correlation with egg quality, embryo quality and the outcome of pregnancy were assessed.【Results】 Total Gonadotropin (Gn) , Gn duration , good embryo rate and cycle cancellation rate of the two groups had no significant difference (P>0.05); the number of dominant follicles on the day of oocyte retrieval , the number of oocytes obtained and MII oocytes of the observation group were significantly higher than those of the control group (P<0.05);The clinical pregnancy rate of the GH group was 25% , while the control group had no clinical pregnancy. GH level of follicular fluid of the two groups was positively correlated with that of IGF-1 and IGF-1R (R=0.55 and 0.58). GH levels of follicular fluid in the observation group were significantly higher than those in the control group (P<0.05); comparing with the level of IGF-1 and IGF-1R, the difference was not statistically significant (P>0.05).IGF-1R and GH levels with MII follicular fluid were higher than those without MII follicular fluid in the same group (P<0.05), and were higher than that of the control group with MII follicular fluid (P<0.05) ; comparison of IGF-1, IGF-1R without MII follicular fluid in the GH group with those in control group with follicle showed no significant difference (P>0.05),but higher than that of the control group without MII follicular fluid.【Conclusion】 GH addition to DOR patients underwent IVF therapy could increase the level of IGF-1, IGF-1R in follicular fluid,and is conducive to improving follicle maturation and follicle qualities.
禹虹;阳翎;唐慧珍;柳朝华;邓朝晖;陈辉莲;吴晓丽;唐亭亭. 生长激素对卵巢储备功能减低患者卵泡发育的影响[J]. 医学临床研究, 2017, 34(3): 424-427,430.
YU Hong, YANG Ling, TANG Hui-zhen,et al. Effects of Growth Hormone on Follicular Development of Patients with Decreased Ovarian Reserve under In-Vitro Fertilization-Embryo Transfer. JOURNAL OF CLINICAL RESEARCH, 2017, 34(3): 424-427,430.
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