P16、Ki-67在宫颈上皮内瘤样变中的表达及其意义

doi:10.3969/j.issn.1671-7171.2016.10.012

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摘要【目的】探讨P16、Ki-67蛋白在宫颈上皮内瘤样变(CIN)组织中的表达及其意义。【方法】收集本院2013年1月至2016年1月收治的96例疑诊为CIN 的患者宫颈活检标本,依据宫颈活检结果将患者分为CINⅠ组22例、CINⅡ组26例、CIN Ⅲ组28例与良性病变良性病变组20例。比较各组P16、Ki-67蛋白表达情况。【结果】各组p16、Ki-67阳性检出率比较差异有统计学意义(P＜0.05),CINⅡ和CIN Ⅲ组p16、Ki-67阳性检出率均显著高于CINⅠ组(P＜0.05),良性病变组阳性检出率显著低于其他三组(P＜0.05);各组p16和Ki-67阳性表达率比较差异具有统计学意义(P＜0.05),CINⅡ和CIN Ⅲ组阳性检出率均高于CINⅠ组(P＜0.05),CIN Ⅲ组阳性检出率均高于CINⅡ组(P＜0.05),良性病变组无阳性表达;CINⅠ组、CINⅡ和CIN Ⅲ组高危型HPV-DNA的阳性表达率分别为31.82%(7/22)、65.38%(17/26)、85.71%(24/28),各组HPV-DNA的阳性表达率比较差异具有统计学意义(χ²=15.464,P=0.000)。【结论】P16、Ki-67蛋白表达与CIN进展及分级密切相关,提示P16、Ki-67蛋白可作为宫颈病变较可靠的指标。

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	王立宏
	王雯

关键词 ：宫颈上皮内瘤样病变/病理学, 基因, 肿瘤抑制, Ki-67抗原, 活组织检查

Abstract：【Objective】To investigate the clinical significance of Ki-67 and P16 protein expression in the diagnosis of cervical intraepithelial neoplasia. 【Methods】Ninety-six patients with cervical intraepithelial neoplasia (CIN) were selected from our hospital from January 2013 to January 2016. Cervical biopsy specimens of all the patients were collected. According to the results of cervical biopsy, the patients were divided into a CIN Ⅰ group with22 cases, a CIN Ⅱ group with 26 cases, a CIN group with 28 cases and 20 cases of benign lesions. The expressions of P16 and Ki-67 protein in each group were analyzed and compared. 【Results】The difference in positive rates of P16 and Ki-67 were statistically significant (P<0.05); in the CIN Ⅱ and CIN Ⅲ groups the positive rates of P16 and Ki-67 were higher than that of CIN Ⅰ group (P<0.05), while the positive rate of the benign group was significantly lower than those of the other three groups (P<0.05). The positive rates of the CIN Ⅱ and Ⅲ groups were higher than that of the CIN Ⅰ group (P<0.05); the positive rate of CIN Ⅲ group was higher than that of CIN Ⅱ group (P<0.05). No positive expression was detected in the benign lesion group. The positive expressions of high-risk HPV-DNA in CIN Ⅰ, Ⅱ and Ⅲ groups were 31.82% (7/22), 65.38% (17/26) and 85.71% (24/28), respectively, and the differences among each group were statistically significant (χ²=15.464,P=0.000). 【Conclusion】The expression of Ki-67 and P16 protein is closely related to the progression of cervical intraepithelial neoplasia; the combined detection of Ki-67 and P16 can be used as an important method for the diagnosis of cervical intraepithelial neoplasia.

Key words： Cervical Intraepithelial Neoplasia/PA Genes, Tumor Suppressor Ki-67 Antigen Biopsy

收稿日期: 2016-02-22

PACS:

R711.74

引用本文:

王立宏;王雯. P16、Ki-67在宫颈上皮内瘤样变中的表达及其意义[J]. 医学临床研究, 2016, 33(10): 1908-1910.
WANG Li-hong, WANG Wen. Clinical and Pathological Analysis of P16 and Ki-67 in Cervical Intraepithelial Neoplasia. JOURNAL OF CLINICAL RESEARCH, 2016, 33(10): 1908-1910.

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http://www.jcr.net.cn/CN/10.3969/j.issn.1671-7171.2016.10.012 或 http://www.jcr.net.cn/CN/Y2016/V33/I10/1908

[1] Mitra A, Tzafetas M, Lyons D,et al.Cervical intraepithelial neoplasia: screening and management[J].Br J Hosp Med (Lond),2016,77(8):C118-123.
[2] Koizumi K, Fujioka T, Yasuoka T,et al. Clinical investigation of the safety and efficacy of a cervical intraepithelial neoplasia treatment using a hyperthermia device that uses heat induced by alternating magnetic fields[J].Mol Clin Oncol,2016 ,5(2):310-316.
[3] Jonathan S.Berek. 妇科学[M]. 第14版.北京:人民卫生出版社,2008:115-117.
[4] Fu Y, Chen C, Feng S, et al. Residual disease and risk factors in patients with high-grade cervical intraepithelial neoplasia and positive margins after initial conization[J].Ther Clin Risk Manag,2015,11:851-856.
[5] Ye N, Liu C, Shi P. Metabolomics analysis of cervical cancer, cervical intraepithelial neoplasia and chronic cervicitis by 1H NMR spectroscopy[J].Eur J Gynaecol Oncol,2015,36(2):174-180.
[6] Kanthiya K, Khunnarong J, Tangjitgamol S, et al. Expression of the p16 and Ki67 in Cervical Squamous Intraepithelial Lesions and Cancer[J].Asian Pac J Cancer Prev,2016,17(7):3201-3206.
[7] 祝莉. 宫颈上皮内瘤变组织中p16蛋白表达与HR-HPV病毒载量的相关性研究[J].医学临床研究, 2015, 32(4):804-809.
[8] Miyamoto S, Hasegawa J, Morioka M, et al. The association between p16 and Ki-67 immunohistostaining and the progression of cervical intraepithelial neoplasia grade 2[J].Int J Gynaecol Obstet,2016, 134(1):45-48.
[9] 王昱, 邓洋, 李卿. 高危型人乳头瘤病毒联合p16、Ki-67检测对子宫颈上皮内瘤变的诊断价值[J].肿瘤研究与临床, 2015, 27(12):823-829.
[10] 陆春雪,杜丽敏,张淑兰,等.宫颈糜烂与宫颈癌及宫颈上皮内瘤变的关系初探[J].医学临床研究,2007, 26(1):71-75.
[11] Yu LL, Chen W, Lei XQ, et al. Evaluation of p16/Ki-67 dual staining in detection of cervical precancer and cancers: a multicenter study in China[J].Oncotarget, 2016 ,7(16):21181-21189.
[12] 汪勤,解正新,张卫琴. p16、p53和Ki-67蛋白在宫颈上皮内瘤变中的表达及意义[J].临床与实验病理学杂志, 2013, 29(5):551-556.
[13] Ziemke P, Marquardt K. Immunocytochemistry of p16(INK4a) and Ki-67 as adjunctive method for routine gynecological cytology of mild and moderate dysplasia [J].Pathologe,2013 ,34(4):323-328.
[14] 李浓英.阴道镜下活检诊断宫颈上皮内瘤变72例临床分析[J].医学临床研究, 2004, 15(11):99-103.
[15] Wentzensen N, Schwartz L, Zuna RE, et al. Performance of p16/Ki-67 immunostaining to detect cervical cancer precursors in a colposcopy referral population[J].Clin Cancer Res,2012,18(15):4154-4162.