摘要【目的】探讨经体外光化学疗法( extracorporeal photopheresis，ECP)处理后的供者淋巴细胞预输注对小鼠心脏移植后移植物存活的影响。【方法】采用小鼠腹部异位心脏移植模型，供者为BALB/C小鼠，受者为C57BL/6小鼠。实验分为两组：对照组直接行心脏移植手术，ECP组于术前 7 d 分离供者脾脏淋巴细胞，给予ECP处理后回输给受者，移植后观察移植物存活情况；部分受者于术后 7 d 获取脾脏用于流式细胞术检测，移植心脏者行HE染色观察其组织学特点，并用免疫组化法检测组织T淋巴细胞浸润。【结果】ECP组移植心脏存活时间延长（20±4.67 d vs 7.5±0.70 d，P＜0.001），伴有移植物排斥反应减轻，淋巴细胞浸润减少，以及外周效应性T细胞生成减少，CD4+CD25+ Foxp3+调节性T细胞增加。【结论】供者淋巴细胞ECP处理后预输注能延长小鼠移植心脏存活时间，其机制可能在于ECP诱导的凋亡淋巴细胞预输注减少了效应性T细胞的数量，并且增加了调节性T细胞的产生。
Abstract：【Objective】 To investigate the effects of extracorporeal photopheresis（ECP） treated donor lymphocyte infusion on graft survival after murine heart transplantation. 【Methods】Abdominal ectopic heart transplantations were performed using BALB/C mice as donors and C57BL/6 mice as recipients. Mice in the control group did not receive any treatment other than transplantation. For mice in ECP treated group, donor splenocytes suspension was treated by ECP and then injected into recipients via penis vein 7 days before transplantation. The graft survival was assessed by abdominal palpation after transplantation. The spleen were harvested on day 7 for further flow cytometry test; and the H&E staining and immunohistochemistry staining of T lymphocyte of graft tissue was evaluated. 【Results】The graft survival time in the ECP treated group was significantly prolonged compared to the control group（20±4.67 d vs 7.5±0.70 d，P＜0.001）. The frequency of effective T cells in the spleens of recipients in ECP treated group was significantly decreased compared to in the spleens of recipients in the control group. The frequency of CD4+CD25+ Foxp3+ regulatory T cells in the spleen of ECP treated mice was higher than that of control group mice. 【Conclusion】Infusion of ECP treated donor lymphocytes prolongs graft survival after murine heart transplantation, possibly via suppressing effector T cell reaction and induction of regulatory T cell generation.
2016, Vol. 33 
