Observations of Genes Variantions in Primary Adult Ingunial Hernias
Department of General Surgery,Shanghai Ninth People's Hospital,Shanghai Jiaotong University School of Medicine，Hernia and Abdominal Wall Surgery Center of Shanghai Jiaotong University,Shanghai 200011，China
Abstract：【Objective】To explore the genomic variations in adult primary inguinal hernia. 【Methods】Utilizing gene chips to scan transversalis fascia of patient with ingunial hernia, the results were compared with corresponding genomes of control patients. The genes with the highest different regulation degrees were chosen to be further examined using the RTqPCR method. 【Results】From the array analysis performed on the two groups, 1189 mRNAs were found to be expressed differently between the groups877 mRNAs were upregulated and 312 mRNAs were downregulated. Of the mRNAs, MYL9 showed the highest degree of upregulation and MYH1 showed the higheset degree of downregulation. We chose the MYL9 and MYH1 as target genes for further examination with RTqPCR. The consistency between the RTqPCR results and microarray data was shown. 【Conclusion】The occurrence and development of inguinal hernia may be associated with a multitude of genetic variations, but MYL9 and MYH1 may play the most significant roles.
2016, Vol. 33 
