Abstract：【Objective】To explore the expression levels of Thrombospondin1(TSP1) in the serum of patients with hepatic carcinoma, and the application values of TSP1 combined with alphafetoprotein (AFP) in the diagnosis of hepatic carcinoma (HCC). 【Methods】Selected 48 cases of clinically diagnosed HCC patients, 43 cases of hepatic fibrosis, and 40 cases of normal healthy perple as the control group. Detection of TSP1 used the sandwich enzyme immunoassay technique, and detection of AFP used Chemiluminescence. A statistical analysis of TSP1 and AFP was performed to show the differences between groups, and both the sensitivity and the specificity of diagnosis to hepatic carcinoma. The best diagnostic value of TSP1 and AFP was defined by the ROC curve. An analysis of the diagnostic value of combined detection of TSP1 and AFP in hepatic carcinoma was performed. 【Result】1. TSP1 in group of hepatic carcinoma was significantly lower than group of hepatic fibrosis and normal control group. The group of hepatic fibrosis was lower than the group of normal control. AFP in the group of hepatic carcinoma was significantly higher than in the group of hepatic fibrosis and group of normal control. 2. To measure TSP1 respectively, the sensitivity of diagnosis to hepatic carcinoma was 79.17%（38/48) and the specificity was 78.31%（65/83）. 3.To measure AFP respectively, the sensitivity of diagnosis to hepatic carcinomas was 58.33%（28/48） and the specificity was 79.52%（66/83）. 4. The sensitivity of the serial test of TSP1 and AFP to the diagnosis of hepatic carcinoma was 43.75% (21/48) and the specificity was 95.18% (79/83). The sensitivity of the parallel test of TSP1 and AFP to the diagnosis of hepatic carcinoma was 93.75% (45/48) and the specificity was 62.65% (52/83).【Conclusions】The levels of TSP1 in serum can be used as a new marker for hepatic carcinoma and both the sensitivity and the specificity of measuring TSP1, respectively, were high in the diagnosis of hepatic carcinoma. It is very beneficial to the early diagnosis of hepatic carcinoma. Combined detection of TSP1 and AFP can improve the sensitivity and specificity in diagnosis of hepatic carcinoma.
2016, Vol. 33 
