Abstract：【Objective】To observe the effects of mild hypothermia on cerebral ischemic rats and observe the expression of HIF1αand MMP2 in order to explore the neuroprotective mechanisms of mild hypothermia. 【Methods】Seventytwo adult SD rats were divided randomly into three groups: the sham group, ischemia/reperfusion model + normal body temperature group, and the ischemia/reperfusion model + hypothermia group. Every group was analyzed at four time points after ischemia/reperfusion: 1d, 3d, 7d and 14d. The expression of HIF1αand MMP2 was detected at each time point immunohistochemically. 【Results】There was a small fraction of HIF1αand MMP2 positive expression in the sham group. In the normal body temperature group and hypothermia group, we observed that the expression of HIF1αand MMP2 increased at 1d but decreased at 3d,there were significant differences between the two groups, which was shown the positivity in hypothermia group lower than that in normal temperature group at 1d,3d and 7d（P＜0.05). However, 14d after ischemic reperfusion, the differences became minimal to nonexistent（P>0.05）. 【Conclusion】Neuroprotection of mild hypothermia may contribute to the decrease in expression of HIF1α and MMP2 after cerebral ischemia reperfusion.
2016, Vol. 33 
