Impact of 1, 25 dihydroxycholecalciferol on Kidney Injury of Type 2 Diabetic Rats
Department of Geriatrics,First College of Clinical Medical Science,China Three Gorges University,Central People's Hospital of Yichang,Yichang Hubei 443003,China
Abstract：【Objective】To study the impact of 1, 25 dihydroxycholecalciferol on kidney injury of type 2 diabetic rats. 【Methods】30 male Wistar rats were randomly divided into normal diet group (N group, 10 rats) and high fat and high glucose diet group (model group, 20 rats), Type 2 diabetes model group were successfully set and randomly divided into two groups, with 10 rats in each group, diabetes mellitus group (M group) was treated by intraperitoneal injection of 2.5 g/(kg•d) of peanut oil, and D group by intraperitoneal injection 2.5 μg/(kg•d) 1, 25 dihydroxycholecalciferol dissolved in peanut oil. After 8 weeks, blood samples were collected to detect blood glucose, creatinine and urea nitrogen;At the same time, the rats were sacrificed and the kidney tissues were kept.Tumor necrosis factor alpha (TNFα), transforming growth factor beta 1 (TGFβ1) were detected by Blot Western and compared with that in renal tissue.【Results】Blood glucose, urea nitrogen and creatinine in M group and D group were significantly higher than those in N group (P<0.05), but the blood glucose, urea nitrogen and creatinine in D group were significantly lower than that in M group (P<0.05); The levels of TNFα、TGFβ1 in M group and D group were significantly higher than those in N group, while the D group was significantly lower than that in M group (P<0.05).【Conclusion】1, 25 dihydroxycholecalciferol can inhibit expression of TNFα、TGFβ1 of diabetic rats kidney and take protective effect on renal injury in rats with diabetes.
2015, Vol. 32 
