Abstract：【Objective】The aim of this study was to investigate the change of aquaporin4（AQP4）after treatment edaravone and mannitol in ischemic brain edema rats.【Methods】150 adult male Wistar rats were randomly divided into randomized to six groups：normal, false surgery, surgery, mannitol（M ), edaravone（E）, and edaravone combined with mannitol（E with M）．Modified Longa rat brain infarction model (middle cerebral artery occlusion) were applied. The morphological changes of ischemic brain and AQP4 mRNA expression were detected after surgery of middle cerebral artery occlusion and measured treatment at 6h, 1d, 3d, 5d and 7d after each treatment。【Results】Within the ischemic brain, the intercellular space and perivascular space became larger, some neurons showed necrosis phenotype, and the edema was most obvious at 3d and was relieved at 7d. After the treatment with mannitol, the edema was not alleviated and few neurons were seen. In the groups of E and E with M, the cerebral edema was alleviated and more neurons were found seen. After the cerebral infarction, the expression of AQP4 mRNA increased, peaked at 3d, then decreased and returned to almost normal almostly at 7d（P<0.05）. AQP4 mRNA of group M was expressed less at the beginning first time, but then increased rapidly（P<0.05） .The expression of AQP4 in the group E and E with M remained low P<0.05）.【Conclusion】AQP4 plays an important role in the formation of ischemic cerebral edema. The expression of AQP4 mRNA increased after the cerebral infarction increased. Through the treatment of edaravone combined with mannitol, the expression of AQP4 mRNA decreased and the brain edema was alleviated.
李冬梅 张红岩 张立新 高红华 高飞 惠林萍. 依达拉奉与甘露醇联合应用对大鼠脑梗死后脑组织内水通道蛋白4表达的影响[J]. 医学临床研究, 2015, 32(10): 1882-1885.
LI Dongmei,ZHANG Hongyan,ZHANG Lixin,et al. Effect of Edaravone Combined with Mannitol on Expression of AQP4 mRNA After ischemic Brain Edema in Rats. JOURNAL OF CLINICAL RESEARCH, 2015, 32(10): 1882-1885.
2015, Vol. 32 
