当归芍药散对大鼠实验性牙移动后三叉神经脊髓核尾侧亚核区域中cfos和iba1表达的影响

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摘要【目的】探讨当归芍药散对大鼠实验性牙移动后三叉神经脊髓核尾侧亚核（SpVc）区域中致癌因子cfos和小胶质细胞膜特异性标记物iba1表达的影响。【方法】36只SD大鼠随机分为对照组（Sham组），牙齿加力组（ETM组）和当归芍药散（DangguiShaoyaosan ,DSS）处理组（DSS组）；分别检测在加力后不同时间点单位时间空口咀嚼时间（chewinglike jaw movements，VCM）及牙齿移动后1 d各组SpVc内fos和iba1免疫组化及Westernblot蛋白表达的变化。【结果】ETM组大鼠VCM在加力d1时间点达到峰值，随后逐渐下降；和ETM组比较，DSS组在4 h，12 h开始减少（P<0.05），牙移动后d1减少最明显（P<0.01）, ETM组与同一时间点Sham组比较无显著性差异（P>0.05）。Sham组SpVc有很少量cfos，iba1表达，呈阴性或浅染，胞体较小，突起表达弱，呈散在分布，形态不清晰；ETM组免疫阳性细胞表达明显增加，染色加深，胞体变大，形态清晰丰满，且分布区域向SpVc较深层趋向，DSS组SpVc区域仅有很少量cfos，iba1表达，免疫阳性小胶质细胞的胞体小，胞质着色较深，突起弱阳性表达。ETM组cfos，iba1蛋白表达均显著高于Sham组和DSS组，差异有统计学意义（P<0.05）；而Sham组与DSS组比较无显著性差异（P>0.05）。【结论】大鼠实验性后牙移动后局部激活SpVc区域cfos，iba1表达，DSS处理后可通过弱化牙齿移动后大鼠SpVc神经元和胶质细胞的激活反应，可减轻大鼠牙齿移动后自发痛。

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关键词 ：当归△/药理学, 芍药/药理学, 牙移动, 三叉神经核, 原癌基因蛋白质cfos

Abstract：【Objective】To investigate the effect of DangguiShaoyaosan on the expression of the carcinogen cfos and Iba1(the microglia membrane specific marker) in spinal trigeminal subnucleus caudalis after the experimental tooth movement(ETM) on rats.【Methods】36 rats were randomly divided into the sham Group , the ETM Group and the DSS Group （DangguiShaoyaosan ）, Chewinglike jaw movements(VCM) at different time points and Western blot protein expression changes of each SpVc fos and Iba1 immunohistochemistry 1 day after tooth movement were detected respectively.【Results】 The VCM of ETM group reached the peak value 1 day after loading, then decreased gradually. Compared with the ETM group ,the DSS group began to decrease at 4 h, 12 h (P<0.05), the decrease appeared most significantly 1d after tooth movement (P<0.01) and the ETM group had no significant difference with the sham group at the same time point (P>0.05)；SpVc at the Sham group had very little cfos and Iba1 expression, which appeared negative or light staining, small cell body , weak projection expression, and scattered in the distribution whose morphology is unclear.The expression of positive cells in the ETM group significantly increased, the staining deepened, the cell body became larger, the morphology was clear and full, and the distribution of the cells was deeper towards SpVc ; SpVc had only a small amount of cfos and Iba1 expression in the DSS group, and the cellbody of immune positive microglia was smaller and the cytoplasm was darker, while the projection appeared weakly positive.The expression of cfos in ETM group was significantly higher than that in group Sham and DSS, and the difference was statistically significant (P<0.05), while there was no significant difference between the Sham group and the DSS group (P>0.05).【Conclusion】Experimental tooth movement on rats is able to locally activate the expression of cfos and Iba1 in SpVc area , and DSS treatment can weaken activation of SpVc neurons and glial cells by tooth remove and reduce their spontaneous pain caused by tooth moving.

Key words： ANGELICA SINENSIS△/PD PAEONIA VEITCHII/PD Tooth Movement Trigeminal Nuclei ProtoOncogene Proteins cfos

收稿日期: 2015-02-03

基金资助:国家自然科学基金（No:30772450）

通讯作者: 通讯作者，刘洪臣Email：liuhongcheng301@hotmail.com

引用本文:

栗洪师李冬霞冯岩顾欣刘洪臣. 当归芍药散对大鼠实验性牙移动后三叉神经脊髓核尾侧亚核区域中cfos和iba1表达的影响[J]. 医学临床研究, 2015, 32(10): 1879-1885.
LI Hongshi,LI Dongxia,FENG Yan,et al. Effect Analysis of DangguiShaoyaosan on Cfos and Iba1 Expression in Spinal Trigeminal Subnucleus Caudalis after the Rat Experimental Tooth Movement. JOURNAL OF CLINICAL RESEARCH, 2015, 32(10): 1879-1885.

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