Abstract:【Objective】 To investigate the target monitoring and risk factors of nosocomial infection in ICU, and to put forward the preventive measures specifically to control nosocomial infection. 【Methods】 1,289 patients in ICU admitted to the hospital from 1st January 2013 to 31st December 2013 were chosen to calculate the average severity of illness (ASIS), the adjusted incidence of nosocomial infection, the invasive operation related infections and other relevant indicators. High risk factors for infection were analyzed . 【Results】 ICU patients with nosocomial infection covered 66 cases (5.12%), the daily incidence of infection rate was1.036% . After adjustment the infection rate was 1.98‰. The quarterly hospital infection showed a downward trend. The average severity of illness was 2.58, and the incidence of nosocomial infection was 1.98% after adjustment of the severity of the disease,;The most common infections were those related invasive operation : ventilator associated with pneumonia, vascular catheter related bloodstream infection , and catheter related urinary tract infection. The pathogens were mainly gram negative bacteria, with a total of 183 strains (90.59%),and gram positive bacteria took 19 (9.41%). 【Conclusion】 The infection rate of ICU was so high mainly because of 3 kinds of invasive operation related infections; Dynamic changes in ICU infection information can timely be accurated through the access of target monitoring so as to develop interventions to reduce the incidence of nosocomial infection in ICU.