摘要目的探讨变应性鼻炎（allergic rhinitis ，AR）鼻黏膜组织重塑特点并检测与组织重塑相关的白细胞介素（IL ）‐6在A R患者鼻黏膜组织中的表达及意义。方法健康对照组15例、A R组25例（单纯A R患者15例，伴哮喘AR 10例）。取受试者中鼻甲黏膜组织，苏木素伊红（HE）染色法观察嗜酸性粒细胞浸润并测定上皮损伤情况；阿辛蓝‐过碘酸‐希夫（AB‐PAS）染色法计数杯状细胞数；三色胶原（MT）染色测定细胞外基质沉积面积百分比。酶联免疫吸附试验（ELISA）测定组织中IL‐6表达情况。结果①AR组嗜酸性粒细胞浸润、杯状细胞化生、上皮细胞损伤、胶原沉积及IL‐6表达均较对照组明显增多（ P ＜0．05）。②伴发哮喘的AR组嗜酸性粒细胞浸润、胶原沉积、IL‐6表达比单纯AR组增多更为明显（ P ＜0．05），但是杯状细胞化生、上皮细胞损伤3级无明显差异。结论 A R的鼻黏膜组织发生了重塑，IL‐6积极参与了A R鼻黏膜组织的重塑。哮喘可能会影响上呼吸道组织结构重塑的发生、发展。
Abstract：[Objective]To explore the nasal mucosa remodeling and determine the changes in expression of interleukin‐6 (IL‐6) in patients with allergic rhinitis (AR) .[Methods]A total of 40 middle turbinate tissues samples were obtained from 15 healthy subjects ,15 AR patients and 10 AR asthmatics .The infiltration of eo‐sinophils in nasal mucosa and the damage of epithelium were examined by hematoxylin and eosin staining (HE) .Goblet cell hyperplasia was counted by alcian blue‐periodic acid‐Schiff (AB‐PAS) double staining .The extracellular matrix (ECM) was stained by masson's trichrome (MT) .And the percentage of stained EMC ar‐ea was processed by the software and transferred into Microsoft Excel for statistical analyses .The level of in‐terleukin 6 (IL‐6) in middle turbinate tissues was measured by enzyme‐linked immunosorbent Assay (ELISA) .[Results]The eosinophil infiltration ,goblet cells ,epithelial damage ,ECM deposition and level of IL‐6 signifi‐cantly increased in AR patients versus control group ( P < 0 .05) .Yet the extent of eosinophil infiltration , ECM deposition and the expression of IL‐6 were much higher in AR asthmatics than those of AR non‐asthmat‐ics ( P<0 .05) .[Conclusion] The remodeling of nasal mucosa is evident in AR patients .And IL‐6 plays some roles during tissue remodeling .Furthermore asthma may influence upper airway remodeling .
基金资助:上海市普陀区卫生局科研课题基金资助(PWG-06-04)
引用本文:
冯会杰%白素娟%李文秀. 变应性鼻炎鼻黏膜组织重塑的特点及白细胞介素-6在鼻黏膜中的表达[J]. 医学临床研究, 2015, 32(1): 35-38.
FENG Hui-jie%BAI Su-juan%LI Wen-xiu. Characterization and Expression of Interleukin-6 in Remodeling of Nasal Mucosa in Allergic Rhinitis. JOURNAL OF CLINICAL RESEARCH, 2015, 32(1): 35-38.
2015, Vol. 32 
