摘要【目的】探讨基质金属蛋白酶-9（MMP-9）、人白细胞分化抗原-34（CD34）在膀胱尿路上皮癌中的表达及意义。【方法】应用免疫组织化学法（S-P法），检测60例膀胱尿路上皮癌组织及15例正常膀胱组织中MMP-9表达及CD34标记的肿瘤微血管密度（MVD）值。【结果】①膀胱尿路上皮癌组织中MMP-9的阳性表达率为58．33％（35/60），CD34标记M VD平均值为20．91±8．83，明显高于正常膀胱组织的6．67％（1/15）和9．49±2．73，且两组相比差异均有显著性（P ＜0．01）。②在膀胱尿路上皮癌组织中MMP-9的阳性表达率和CD34标记M VD平均值与肿瘤组织学分化程度，浸润深度（临床分期），肿瘤大小有相关（ P ＜0．05），与单发或多发、年龄、性别无关（ P＞0．05）。③在M M P-9的阳性表达的病例中，CD34标记M VD值随M M P-9表达强度的增加而增加，两者呈明显的正相关（ P ＜0．01）。【结论】M M P-9的表达及CD34标记M VD值在膀胱尿路上皮癌中的表达明显升高，可作为判断膀胱尿路上皮癌恶性程度及预后的重要参考指标。
Abstract：To explore the expression of matrix metalloproteinase-9(MMP-9) and human leuko-cyte differentiation antigen-34(CD34) in bladder urothelial carcinoma and its significance .[Methods] The ex-pression of MMP-9 and CD34-labelled microvessel density (MVD) in 60 cases of bladder urothelial carcinoma and 15 cases of normal bladder were measured by S-P immunohistochemical method .[Results]The expression rate of MMP-9 and the average CD34-labelled MVD in bladder urothelial carcinoma were 58 .33% (35/60) and 20 .91 ± 8 .83 respectively ,which were obviously higher than those in normal bladder tissues [6 .67% (1/15) and (9 .49 ± 2 .730 ,respectively)] ,and there was significant difference between two groups( P <0 .01) .The expression rate of MMP-9 and the average CD34-labelled MVD in bladder urothelial carcinoma were correlated with the degree of histological differentiation ,invasion depth(clinical stage) and tumor size( P <0 .05) ,but not correlated with single or multiple tumor ,age and sex( P > 0 .05) .The CD34-labelled MVD in patients with positive expression of MMP-9 was increased with the intensity of MMP-9 expression ,and there was posi-tive correlation between them ( P <0 .01) .[Conclusion] MMP-9 expression and CD34-labelled MVD in blad-der urothelial carcinoma are increased obviously ,and can be used to be the important indicators of malignancy and prognosis of bladder urothelial carcinoma .
2014, Vol. 31 
