Abstract：[Objective]To construct fusion protein expression vector pEGFP-ap2-AQP7 of promoter/enhancer sequences of ap2 and AQP7 cDNA.[Methods]The products of splicing promoter/enhancer of ap2 and AQP7 cDNA were cloned into pIRES2-EGFP.Adenovirus vector recombinant fusion protein was obtained.The infected 3T3 fat cell was used to determine the infextation effect.[Results]① The pEGFP-ap2-AQP7 fusion protein was expressed in the constructed cell strains stably.The enhanced green fluorescent protein was used as a marker.The positio-ning of AQP7 in 3T3 fat cells were observed.[Conclusion]The stable construction of 3T3 fat cell strains express-ing fusion protein pEGFP-ap2-AQP7 can provide the basis for intracellular localization and function research of AQP7 .
基金资助:广东省自然科学基金项目(8451803302001917)
引用本文:
白晓苏%陆泽元%邵豪. 含有ap2启动子／增强子、AQP7 cDNA的腺病毒载体的构建及鉴定[J]. 医学临床研究, 2014, 31(5): 856-858.
BAI Xiao-su%LU Ze-yuan%SHAO Hao. Construction and Identification of Adenovirus Vector of Promoter/Enhancer Sequences of ap2 and AQP7 cDNA. 医学临床研究, 2014, 31(5): 856-858.
2014, Vol. 31 
