Abstract：[Objective]To explore the effect of RNA interference(RNAi)targeting XIAP gene on cell ap-optosis of human endometrial carcinoma.[Methods]Specific small interference RNA(siRNA)of XIAP was de-signed and composed.Endometrial cancer cell line RL95-2 was transfected.The changes of mRNA and protein of XIAP after transfection were detected by real time RT-PCR and Western Blotting,respectively.MTT and flow cytometry method were used to detect cell proliferation and apoptosis.[Results]After transfection of XI-AP siRNA,the relative fold value of mRNA transcription volume and the relative protein expression volume in specific transfection group were (0.04±0.06)and (0.590±0.178)respectively,which were obviously lower than those in the control group(P<0.05).The cell growth inhibition rate in specific transduction group was (47.86±4.46)%,which was obviously higher than that in control group(P<0.05).[Conclusion]The ex-periment in vitro indicates that the composed siRNA can effectively inhibit the transcription and expression of XIAP in human endometrial cancer cell line RL95-2,and further promote cell apoptosis of endometrial carcino-ma significantly.The mechanism of promoting cell apoptosis still needs further investigation.
2014, Vol. 31 
