摘要【目的】探讨缺氧诱导因子-1α（HIF-1α），血管内皮生长因子（VEGF）在喉癌中的表达及与临床病理特征的关系。【方法】采用免疫组化染色检测62例喉癌组织及46例癌旁组织中HIF-1α和VEGF的表达情况，并结合相关临床病理参数进行分析。【结果】HIF-1α和VEGF在喉癌中阳性表达率分别为67．74％和62．90％，均高于癌旁组织中的表达（ P ＜0．05），且两者表达呈显著正相关性（ P ＜0．05）。HIF-1α表达与TNM 分期和临床分期相关，VEGF表达则与临床分期和淋巴结转移相关（ P ＜0．05）。生存分析表明 HIF-1α、VEGF阳性和阴性表达患者无瘤生存率和总生存率差异显著（ P ＜0．05）。【结论】HIF-1α和VEGF在喉癌的发生发展中发挥着重要作用，且两者存在相互协同作用。HIF-1α可能通过上调VEGF的表达，促进肿瘤血管生成，并导致肿瘤浸润和淋巴结转移，两者可作为判断喉癌生物学行为和预后的重要指标。
Abstract：[Objective] To explore the expression of hypoxia-inducible factor-1α(HIF-1α) and vascular endothelial growth factor(VEGF)in laryngeal carcinoma and its clinical significance .[Methods] Immunohistochemical method was used to detect the expression of HIF-1αand VEGF in 62 cases of laryngeal carcinoma tissue and 46 cases of adjacent non-cancerous tissue .Clinicopathological parameters were also analyzed .[Results]The expression rates of HIF-1αand VEGF in laryngeal carcinoma tissue were 67 .74% and 62 .90% respectively ,which were higher than those in adjacent noncan-cerous tissue( P<0 .05) ,and there was a positive correlation between them ( P<0 .05) .The expression of HIF-1αwas associated with T stage and clinical stage ,and the expression of VEGF was associated with clinical stage and lymph node metastases( P<0 .05) .Survival analysis showed that there were significant differences in disease-free survival and overall survival between patients with positive expression of HIF-1αor VEGF and patients with negative expression ( P<0 .05) .[Conclusion] The expression of HIF-1αand VEGF may play an important role in the occurrence and development of la-ryngeal carcinoma ,and they have synergetic effect .HIF-1αmay promote tumor angiogenesis which leads to tumor inva-sion and lymph node metastasis by up-regulating VEGF expression .Both HIF-1αand VEGF can be used as important in-dicators to assess the biological behavior and prognosis of laryngeal carcinoma .
2014, Vol. 31 
